Wearing a black suit and red tie, and with Melania dressed in white, and accompanied by his younger daughter Tiffany and the couple's son Barron, the first family visited the Bethesda-by-the-Sea church on Sunday morning, Efe news reported.
Donald Trump and Melania were married in this church in 2005 and have observed other important holidays there.
Also with the group, which entered the Gothic style church by a side door, were Melania's parents.
A nearby street was closed to vehicular traffic starting early Sunday morning, the South Florida daily Sun Sentinel reported, publishing a photo of the president greeting the public near Bethesda-by-the-Sea, which opened its doors for Mass at 10.30 a.m.
Other people attending the service had to pass through a metal detector at the entrance and later, inside the church, Trump gave a thumbs-up to some of them.
At about 12.15 p.m., shortly before the service ended, Trump and his family left the church to take part in the traditional Easter egg hunt.
Joining the president at Mar-a-Lago mansion were his sons Eric and Donald Jr., and their families.
Earlier on Sunday morning, Trump sent out a "Happy Easter to everyone!" message on Twitter.
Trump and the First Lady will participate in the traditional White House Easter Egg Roll on Monday, which Melania will host in a century-old tradition to be attended by thousands of children.